| Field | Specification |
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| Alternative Names | 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6, 5-HT-6, 5HT6R |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
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| Target |
Overview
Anti-5HT6 Receptor (HTR6) (extracellular) Antibody is an antibody targeting 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6, 5-HT-6, 5HT6R Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6, 5-HT-6, 5HT6R (also reported as 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6, 5-HT-6, 5HT6R).
- Immunogen/epitope region: Extracellular, 2nd loop..
- Homology note: Rat - 14 out of 15 amino acid residues identical Mouse - 13 out of 15 amino acid residues identical The antibody is not recommended for human samples (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
5HT6 also known as 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6, or 5-HT-6, is a member of the serotonin (5-HT) family of receptors that comprises 14 members, classified into 7 distinct families (5HT1 to 5HT7). With the exception of 5HT3 that is a ligand-gated channel, all other family members are G protein-coupled receptors (GPCRs) 1,2.The 5HT6 receptor is almost exclusively present in the central nervous system and is distributed in various brain regions, including the striatum, nucleus accumbens, hippocampus, thalamus, cerebellum, dentate gyrus, olfactory tubercles, amygdala, and cerebral cortex.1,2,5Based on the unique expression pattern of 5HT6 in the brain, research on the 5HT6 receptor has suggested its involvement in various neurological and psychiatric conditions, including mood disorders, schizophrenia, cognitive function and Alzheimer's disease 1,2,5. Indeed several antagonists of 5HT6 receptor have been examined in the context of schizophrenia3, Alzheimer's disease4 and cognitive impairment 5.
Research relevance and current trends
- Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
- Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
- Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-SR038.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-SR038; Negative control: BLP-SR038.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
