| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-binding cassette sub-family G member 5;Sterolin-1;ABCG5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ATP-binding cassette sub-family G member 5 (gene ABCG5) in human samples and is validated for Western blot. The predicted molecular weight is 72.5 kDa, and the supplier reports an observed band at about 73 kDa.
ABCG5 forms an obligate heterodimer with ABCG8 that pumps cholesterol and plant sterols out of intestinal cells and liver cells into the gut lumen and bile. The complex limits dietary sterol absorption, and mutations in either gene cause sitosterolemia.
| Target | ATP-binding cassette sub-family G member 5 (gene ABCG5; UniProt Q9H222, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 72.5 kDa |
| Observed MW | 73 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): MCF-7 cells, A549 cells, PANC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human ABCG5. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ATP-binding cassette sub-family G member 5 in the cell membrane and apical cell membrane. Tissue expression noted by UniProt (human): Strongly expressed in the liver, lower levels in the small intestine and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tao-tao Wang et al. (2023) QiShenYiQi pill inhibits atherosclerosis by promoting TTC39B-LXR mediated reverse cholesterol transport in liver. Phytomedicine. 10.1016/j.phymed.2023.155192
Wang Zhiwen et al. (2022) The LOX-1 receptor ectopically expressed in the liver alleviates atherosclerosis by clearing Ox-LDL from the circulation. Molecular Medicine. 10.1186/s10020-022-00450-3
Qing Zhang et al. (2023) Overexpression of LOX-1 in hepatocytes protects vascular smooth muscle cells from phenotype transformation and wire injury induced carotid neoatherosclerosis through ALOX15. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2023.166805
Mingxiao Luo et al. (2024) Hif-1α expression targets the TMA/Fmo3/TMAO axis to participate in gallbladder cholesterol stone formation in individuals living in plateau regions. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2024.167188
