| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ATP-binding cassette sub-family G member 5;Sterolin-1;ABCG5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5, different from the related mouse and rat sequences by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ABCG5 (ATP-binding cassette sub-family G member 5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ABCG5 Antibody Picoband® catalog # PB9415. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5, different from the related mouse and rat sequences by two amino acids.
- Molecular weight context: reported MW: 73 kDa; calculated MW: 72504 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
ATP-binding cassette sub-family G member 5; ATP-binding cassette sub-family G member 5. ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb. The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8. The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype. Functional note: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Reported localization: Membrane ; Multi-pass membrane protein . Expression/tissue context: Strongly expressed in the liver, lower levels in the small intestine and colon.
Research relevance and current trends
- Atherosclerosis: Researchers commonly examine how ABCG5 (ATP-binding cassette sub-family G member 5) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how ABCG5 (ATP-binding cassette sub-family G member 5) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how ABCG5 (ATP-binding cassette sub-family G member 5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ABCG5 (ATP-binding cassette sub-family G member 5) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
