| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 1-acylglycerol-3-phosphate O-acyltransferase ABHD5;2.3.1.51;Abhydrolase domain-containing protein 5;Lipid droplet-binding protein CGI-58;ABHD5;NCIE2;CGI-58; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Abhd5 recombinant protein (Position: R169-D349). Human Abhd5 shares 96.7% amino acid (aa) sequence identity with both mouse and rat Abhd5. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Abhd5 Antibody Picoband® catalog # PB10021. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Abhd5 recombinant protein (Position: R169-D349). Human Abhd5 shares 96.7% amino acid (aa) sequence identity with both mouse and rat Abhd5. (reported region: R169-D349).
- Molecular weight context: reported MW: 43 kDa; calculated MW: 39096 MW
- Reactivity: Human,Rat
- Applications: IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
1-acylglycerol-3-phosphate O-acyltransferase ABHD5; 1-acylglycerol-3-phosphate O-acyltransferase ABHD5. 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 is an enzyme that in humans is encoded by the ABHD5 gene. The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated withChanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. Functional note: Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis (PubMed:18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289). Involved in keratinocyte differentiation (PubMed:18832586). Regulates lipid droplet fusion (By similarity). . Reported localization: Cytoplasm . Lipid droplet . Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity). . Expression/tissue context: Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level). .
Research relevance and current trends
- Cancer: Researchers commonly examine how ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
