| Field | Specification |
|---|---|
| Target | |
| Alternative names | Very long-chain specific acyl-CoA dehydrogenase, mitochondrial;VLCAD;1.3.8.9;ACADVL;VLCAD; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ACADVL, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects very long-chain acyl-CoA dehydrogenase, mitochondrial (gene ACADVL) in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 70.4 kDa, and the supplier reports an observed band at about 70 kDa.
Very long-chain acyl-CoA dehydrogenase (ACADVL, VLCAD) is bound to the inner mitochondrial membrane and starts beta-oxidation of long-chain fatty acids. This pathway is a major energy source for heart and muscle, and VLCAD deficiency is a recognized inherited metabolic disorder.
| Target | Very long-chain acyl-CoA dehydrogenase, mitochondrial (gene ACADVL; UniProt P49748, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 70.4 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells lysate, human SiHa cells lysate, human A431 cells lysate, human U251 cells lysate, rat liver tissue lysates, rat heart tissue lysates, mouse liver tissue lysates, mouse heart tissue lysates.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human ovarian cancer tissue, rat heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed result (IP): HeLa cells.
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Synthetic peptide from the C-terminal region of human ACADVL. It differs from the mouse sequence at three residues and from the rat sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places very long-chain acyl-CoA dehydrogenase, mitochondrial in the mitochondrion inner membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Predominantly expressed in heart and skeletal muscle (at both mRNA and protein levels). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Lei et al. (2022) Notoginsenoside R1 Regulates Ischemic Myocardial Lipid Metabolism by Activating the AKT/mTOR Signaling Pathway. Frontiers in Pharmacology. 10.3389/fphar.2022.905092
