Anti-ACADVL Antibody Picoband®

Overview

This antibody is intended for detection of ACADVL (Very long-chain specific acyl-CoA dehydrogenase, mitochondrial) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-ACADVL Antibody Picoband® catalog # PB10023. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human ACADVL, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
• Molecular weight context: reported MW: 70 kDa; calculated MW: 70390 MW
• Reactivity: Human,Mouse,Rat
• Applications: Flow Cytometry, IP, IF, IHC, ICC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Very long-chain specific acyl-CoA dehydrogenase, mitochondrial; Very long-chain specific acyl-CoA dehydrogenase, mitochondrial. Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (VLCAD) is an enzyme that in humans is encoded by the ACADVL gene. The protein encoded by this gene is targeted to the inner mitochondrial membrane, where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenaseis specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. Functional note: Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accommodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons. . Reported localization: Mitochondrion inner membrane.

Research relevance and current trends

• Cancer: Researchers commonly examine how ACADVL (Very long-chain specific acyl-CoA dehydrogenase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
• Cancer Metabolism: Researchers commonly examine how ACADVL (Very long-chain specific acyl-CoA dehydrogenase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
• Cardiovascular: Researchers commonly examine how ACADVL (Very long-chain specific acyl-CoA dehydrogenase, mitochondrial) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative ACADVL (Very long-chain specific acyl-CoA dehydrogenase, mitochondrial) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of ACADVL (Very long-chain specific acyl-CoA dehydrogenase, mitochondrial) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
• Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.