| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; T2; ACAT1; ACAT; MAT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACAT1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29A55 is a rabbit monoclonal antibody that recognizes acetyl-CoA acetyltransferase, mitochondrial (gene ACAT1) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 45.2 kDa, and the supplier reports an observed band at about 40 kDa.
Mitochondrial acetyl-CoA acetyltransferase (ACAT1, T2) is a thiolase that acts in the final step of beta-oxidation and in ketone body metabolism and isoleucine breakdown. Its deficiency, beta-ketothiolase deficiency, causes episodes of ketoacidosis.
| Target | Acetyl-CoA acetyltransferase, mitochondrial (gene ACAT1; UniProt P24752, human) |
|---|---|
| Clone | 29A55 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.2 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human Caco-2 cells, human PC-3 cells, human K562 cells, rat lung tissue, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ACAT1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places acetyl-CoA acetyltransferase, mitochondrial in the mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
