| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuronal acetylcholine receptor subunit alpha-4; CHRNA4; NACRA4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACHA4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23C95 is a rabbit monoclonal antibody that recognizes neuronal acetylcholine receptor subunit alpha-4 (gene CHRNA4) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 70.0 kDa, and the supplier reports an observed band at about 70 kDa.
The neuronal acetylcholine receptor alpha-4 subunit (CHRNA4) combines with beta-2 subunits to form the most abundant high-affinity nicotinic receptor in the brain. These ligand-gated cation channels mediate many effects of nicotine, and CHRNA4 variants are associated with a familial form of nocturnal frontal lobe epilepsy.
| Target | Neuronal acetylcholine receptor subunit alpha-4 (gene CHRNA4; UniProt P43681, human) |
|---|---|
| Clone | 23C95 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 70.0 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ACHA4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neuronal acetylcholine receptor subunit alpha-4 in the synaptic cell membrane and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
