| Field | Specification |
|---|---|
| Target | |
| Alternative names | Long-chain-fatty-acid--CoA ligase 3;6.2.1.3;Long-chain acyl-CoA synthetase 3;LACS 3;ACSL3;ACS3, FACL3, LACS3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ACSL3 recombinant protein (Position: M12-I224). Human ACSL3 shares 91.1% and 90.1% amino acid (aa) sequence identity with mouse and rat ACSL3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes fatty acid CoA ligase Acsl3 in human samples and is validated for Western blot. The predicted molecular weight is 80.4 kDa, and the supplier reports an observed band at about 80 kDa.
Long-chain acyl-CoA synthetase 3 (ACSL3) activates long-chain fatty acids to acyl-CoAs, mainly for building lipids. It is located on the ER and lipid droplets, supports phosphatidylcholine synthesis for lipoprotein secretion, and is studied in hepatic lipogenesis and lipid droplet biology.
| Target | Fatty acid CoA ligase Acsl3 (gene ACSL3; UniProt O95573, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 80.4 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human ACSL3 fragment (Met12–Ile224), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91.1% and 90.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places fatty acid CoA ligase Acsl3 in the mitochondrion outer membrane, peroxisome membrane, microsome membrane and endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
