| Field | Specification |
|---|---|
| Target | |
| Alternative names | Activin receptor type-1; Activin receptor type I; ACTR-I; Activin receptor-like kinase 2; ALK-2; Serine/threonine-protein kinase receptor R1; SKR1; TGF-B superfamily receptor type I; TSR-I; ACVR1; ACVRLK2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACVR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A99 is a rabbit monoclonal antibody that recognizes activin receptor type-1 (gene ACVR1) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 57.2 kDa, and the supplier reports an observed band at about 57 kDa.
Activin receptor type-1 (ACVR1, ALK2) is a type I serine/threonine kinase receptor for bone morphogenetic proteins (BMPs). With type II receptors it phosphorylates SMAD1/5/8 to control bone, heart and nervous system development, and activating mutations cause fibrodysplasia ossificans progressiva and occur in diffuse intrinsic pontine glioma.
| Target | Activin receptor type-1 (gene ACVR1; UniProt Q04771, human) |
|---|---|
| Clone | 25A99 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.2 kDa |
| Observed MW | 57 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ACVR1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places activin receptor type-1 in the membrane. Tissue expression noted by UniProt (human): Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiang Wang et al. (2017) Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma. Oncotarget. 10.18632/oncotarget.15222
