| Field | Specification |
|---|---|
| Target | |
| Alternative names | A disintegrin and metalloproteinase with thrombospondin motifs 13;ADAM-TS 13;ADAM-TS13;ADAMTS-13;3.4.24.87;von Willebrand factor-cleaving protease;vWF-CP;vWF-cleaving protease;ADAMTS13;C9orf8;UNQ6102/PRO20085; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ADAMTS13 recombinant protein (Position: A299-R488). Human ADAMTS13 shares 85.2% amino acid (aa) sequence identity with mouse ADAMTS13. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects A disintegrin and metalloproteinase with thrombospondin motifs 13 (gene ADAMTS13) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 153.6 kDa; the supplier reports observed bands at 154, 200 kDa.
ADAMTS13 is a plasma metalloprotease that cuts ultra-large von Willebrand factor (vWF) multimers into smaller forms, limiting platelet clumping. Severe ADAMTS13 deficiency, inherited or autoimmune, causes thrombotic thrombocytopenic purpura (TTP).
| Target | A disintegrin and metalloproteinase with thrombospondin motifs 13 (gene ADAMTS13; UniProt Q76LX8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 153.6 kDa |
| Observed MW | 154, 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA (capture antibody) | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human hepatocellular carcinoma paracancerous tissue, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human ADAMTS13 fragment (Ala299–Arg488), expressed in E. coli. Sequence identity with the mouse ortholog: 85.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes A disintegrin and metalloproteinase with thrombospondin motifs 13 as a secreted protein. Tissue expression noted by UniProt (human): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
