| Field | Specification |
|---|---|
| Target | |
| Alternative names | A disintegrin and metalloproteinase with thrombospondin motifs 13;ADAM-TS 13;ADAM-TS13;ADAMTS-13;3.4.24.87;von Willebrand factor-cleaving protease;vWF-CP;vWF-cleaving protease;ADAMTS13;C9orf8;UNQ6102/PRO20085; |
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| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ADAMTS13 recombinant protein (Position: A299-R488). Human ADAMTS13 shares 85.2% amino acid (aa) sequence identity with mouse ADAMTS13. |
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Overview
This antibody is intended for detection of ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ADAMTS13 Antibody Picoband® catalog # PB10028. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human ADAMTS13 recombinant protein (Position: A299-R488). Human ADAMTS13 shares 85.2% amino acid (aa) sequence identity with mouse ADAMTS13. (reported region: A299-R488).
- Molecular weight context: reported MW: 154 kDa; calculated MW: 153604 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
A disintegrin and metalloproteinase with thrombospondin motifs 13; A disintegrin and metalloproteinase with thrombospondin motifs 13. ADAMTS13 (a disintegrin and metalloproteinase with athrombospondin type 1 motif, member 13), also known as VWFCP, is a zinc-containing metalloprotease enzyme that cleaves von Willebrand factor (vWf), a large protein involved in blood clotting. It is secreted in blood and degrades large vWf multimers, decreasing their activity. This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. This gene is mapped to 9q34. Functional note: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. . Reported localization: Secreted . Secretion enhanced by O-fucosylation of TSP type-1 repeats. Expression/tissue context: Plasma. Expressed primarily in liver. .
Research relevance and current trends
- Cancer: Researchers commonly examine how ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) relates to this theme using model systems and orthogonal readouts.
- ECM Enzymes: Researchers commonly examine how ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
