| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alcohol dehydrogenase 4;1.1.1.1;Alcohol dehydrogenase class II pi chain;ADH4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human ADH4 recombinant protein (Position: K218-F380). Human ADH4 shares 68.7% and 71.8% amino acid (aa) sequence identity with mouse and rat ADH4, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ADH4 (Alcohol dehydrogenase 4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ADH4 Antibody Picoband® catalog # PB10030. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human ADH4 recombinant protein (Position: K218-F380). Human ADH4 shares 68.7% and 71.8% amino acid (aa) sequence identity with mouse and rat ADH4, respectively. (reported region: K218-F380).
- Molecular weight context: reported MW: 40 kDa; calculated MW: 40 kDa
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Alcohol dehydrogenase 4; Alcohol dehydrogenase 4. Alcohol dehydrogenase 4 is an enzyme that in humans is encoded by the ADH4 gene. This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. Functional note: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. . Reported localization: Cytoplasm. Expression/tissue context: Plasma. Expressed primarily in liver. .
Research relevance and current trends
- Drug Metabolism: Researchers commonly examine how ADH4 (Alcohol dehydrogenase 4) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how ADH4 (Alcohol dehydrogenase 4) relates to this theme using model systems and orthogonal readouts.
- Metabolism: Researchers commonly examine how ADH4 (Alcohol dehydrogenase 4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ADH4 (Alcohol dehydrogenase 4) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
