| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;Afp; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human alpha-fetoprotein. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody is an antibody targeting AFP. Common applications include IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: C3; isotype: Mouse IgG2a; reactivity: Human; observed MW: 43 kDa; calculated MW: 68386 MW.
Boster Bio Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody catalog # MA1001. Tested in IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: AFP — Alpha-fetoprotein
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: C3; Isotype: Mouse IgG2a
- Species reactivity: Human
- Molecular weight guidance: Observed: 43 kDa; Calculated: 68386 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Binds estrogens, fatty acids and metals.
Scientific background (datasheet): Alpha-fetoprotein (AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk (1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.
Cellular localization (datasheet): Isoform 1: Secreted.
Tissue details (datasheet): Plasma. .
Sequence similarities (datasheet): Belongs to the ALB/AFP/VDB family.
Research relevance and current trends
- Commonly studied in contexts related to Serum Proteins,Tumor Antigens,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
