Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody

SKU:BHA21006491
Research Validated
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-AFP antibody (Mouse monoclonal, clone C3, Mouse IgG2a). Validated for immunohistochemistry. Reactivity: Human. Recognizes alpha-fetoprotein. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target AFP
Clone number C3
Host Mouse
Reactivity Human
Isotype Mouse IgG2a
Application(s) IHC
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Options selector
Catalog no. Size Conjugation
MA1001 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated
      Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
      Form: Lyophilized
      Applications: IHC
      Application details: Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target AFP
Alternative names Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;Afp;
Gene ID Human(174), Mouse(11576), Rat(24177)
UniProt # P02773
Host Mouse
Clone C3
Clonality
  • Monoclonal
Isotype
  • Mouse IgG2a
Reactivity
  • Human
Applications
  • Immunohistochemistry
Immunogen Human alpha-fetoprotein.
Purification Ascites
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Cellular localization Isoform 1: Secreted.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MA1001
Main SKU BHA21006491
Mouse Monoclonal

Product Overview

Clone C3 is a mouse monoclonal antibody that recognizes alpha-fetoprotein (gene Afp) in human samples and is validated for IHC.

Alpha-fetoprotein (AFP) is the major serum protein of the fetus, produced mainly by the fetal liver and yolk sac and related in structure to albumin. It binds fatty acids, metal ions and bilirubin, and its levels are widely measured in studies of fetal development and liver tumors such as hepatocellular carcinoma.

Target Alpha-fetoprotein (gene Afp; UniProt P02773, rat)
Clone C3
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG2a
Reactivity Human
Purification Ascites
Form Lyophilized
Formulation 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers
Reconstitution 100 µg/mL after adding 1 mL PBS
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 2–4 µg/mL (Human)

Immunogen

Human alpha-fetoprotein.

Reactivity Notes

The supplier lists reactivity with human. Tissue expression noted by UniProt (rat): Plasma.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 2–4 µg/mL (Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on feline samples?
A.Reactivity with feline samples has not been tested by the supplier. Aligning the immunogen sequence with the feline ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Human alpha-fetoprotein.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Chenyang Wang et al. (2025) Gold nanorod in silver tetrahedron: Cysteamine mediated synthesis of SERS probes with embedded internal markers for AFP detection. Analytica Chimica ACTA. 10.1016/j.aca.2025.343667

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