| Field | Specification |
|---|---|
| Target | |
| Alternative names | ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; CD antigen CD246; ALK |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ALK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20A85 is a rabbit monoclonal antibody that recognizes ALK tyrosine kinase receptor in human, mouse and rat samples and is validated for IHC and ICC/IF. The predicted molecular weight is 176.4 kDa, and the supplier reports an observed band at about 176 kDa.
Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase expressed mainly in the developing nervous system. Chromosomal rearrangements that create ALK fusion proteins, such as NPM-ALK and EML4-ALK, and activating ALK mutations drive anaplastic large cell lymphoma, lung cancer and neuroblastoma, making ALK an important cancer research and drug target.
| Target | ALK tyrosine kinase receptor (gene ALK; UniProt Q9UM73, human) |
|---|---|
| Clone | 20A85 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 176.4 kDa |
| Observed MW | 176 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human ALK.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ALK tyrosine kinase receptor in the cell membrane. Tissue expression noted by UniProt (human): Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
