| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-1-acid glycoprotein 1;AGP 1;Orosomucoid-1;OMD 1;ORM1;AGP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ORM1 recombinant protein (Position: Q19-S201). Human ORM1 shares 47.5% and 48.1% amino acid (aa) sequence identity with mouse and rat ORM1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes alpha-1-acid glycoprotein 1 (gene ORM1) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 23.5 kDa, and the supplier reports an observed band at about 40–47 kDa.
Alpha-1-acid glycoprotein 1 (AGP, orosomucoid, ORM1) is a heavily glycosylated plasma protein made mainly by the liver. It is a major acute-phase protein whose levels rise during inflammation, it binds many basic and neutral drugs, and it is studied as an inflammation marker and in drug binding.
| Target | Alpha-1-acid glycoprotein 1 (gene ORM1; UniProt P02763, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 23.5 kDa |
| Observed MW | 40–47 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human hcct tissue, human hccp tissue, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ORM1 fragment (Gln19–Ser201), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 47.5% and 48.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes alpha-1-acid glycoprotein 1 as a secreted protein. Tissue expression noted by UniProt (human): Expressed by the liver and secreted in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
