| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-1-antitrypsin;Alpha-1 protease inhibitor;Alpha-1-antiproteinase;Serpin A1;Short peptide from AAT;SPAAT;SERPINA1;AAT, PI;PRO0684, PRO2209; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human SERPINA1 recombinant protein (Position: E25-T204). Human SERPINA1 shares 72.8% amino acid (aa) sequence identity with rat SERPINA1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets alpha-1-antitrypsin (gene SERPINA1) in human, monkey and mouse samples and is validated for Western blot. The predicted molecular weight is 46.7 kDa, and the supplier reports an observed band at about 53 kDa.
Alpha-1-antitrypsin (SERPINA1) is an abundant plasma serine protease inhibitor made mainly by the liver, whose main target is neutrophil elastase. By limiting elastase activity it protects lung tissue, and inherited SERPINA1 deficiency variants cause emphysema and liver disease, making it a major subject in respiratory and hepatic research.
| Target | Alpha-1-antitrypsin (gene SERPINA1; UniProt P01009, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.7 kDa |
| Observed MW | 53 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Monkey, Mouse) |
|---|
Samples with a confirmed band (WB): human T47D cells, monkey COS-7 cells, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human SERPINA1 fragment (Glu25–Thr204), expressed in E. coli. Sequence identity with the rat ortholog: 72.8%.
Reactivity Notes
The supplier lists reactivity with human, monkey and mouse. UniProt describes alpha-1-antitrypsin as secreted and also located in the endoplasmic reticulum. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
