| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-1-antitrypsin;Alpha-1 protease inhibitor;Alpha-1-antiproteinase;Serpin A1;Short peptide from AAT;SPAAT;SERPINA1;AAT, PI;PRO0684, PRO2209; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human SERPINA1 recombinant protein (Position: E25-T204). Human SERPINA1 shares 72.8% amino acid (aa) sequence identity with rat SERPINA1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SERPINA1 (Alpha-1-antitrypsin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® catalog # PB10097. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human SERPINA1 recombinant protein (Position: E25-T204). Human SERPINA1 shares 72.8% amino acid (aa) sequence identity with rat SERPINA1. (reported region: E25-T204).
- Molecular weight context: reported MW: 52 kDa; calculated MW: 46737 MW
- Reactivity: Human,Monkey,Mouse
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Alpha-1-antitrypsin; Alpha-1-antitrypsin. SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. Functional note: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Reported localization: Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum. Expression/tissue context: Ubiquitous. Expressed in leukocytes and plasma. .
Research relevance and current trends
- Apoptosis: Researchers commonly examine how SERPINA1 (Alpha-1-antitrypsin) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how SERPINA1 (Alpha-1-antitrypsin) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how SERPINA1 (Alpha-1-antitrypsin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SERPINA1 (Alpha-1-antitrypsin) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
