| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;AFP;HPAFP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets alpha-fetoprotein (gene AFP) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 68.7 kDa, and the supplier reports an observed band at about 69 kDa.
Alpha-fetoprotein (AFP) is the major serum protein of the fetus, produced mainly by the fetal liver and yolk sac and related in structure to albumin. It binds fatty acids, metal ions and bilirubin, and its levels are widely measured in studies of fetal development and liver tumors such as hepatocellular carcinoma.
| Target | Alpha-fetoprotein (gene AFP; UniProt P02771, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 68.7 kDa |
| Observed MW | 69 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human hepatocellular carcinoma tumor tissue (HCCT).
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human AFP fragment (Gln378–Val609), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 71% and 73%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes alpha-fetoprotein as a secreted protein. Tissue expression noted by UniProt (human): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chenyang Wang et al. (2025) Gold nanorod in silver tetrahedron: Cysteamine mediated synthesis of SERS probes with embedded internal markers for AFP detection. Analytica Chimica ACTA. 10.1016/j.aca.2025.343667
Huicui Yang et al. (2011) RPB5-mediating Protein Is Required for the Proliferation of Hepatocellular Carcinoma Cells *. Journal of Biological Chemistry. 10.1074/jbc.M110.136929
Qi Wang et al. (2014) The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP. International Journal of Biological Sciences. 10.7150/ijbs.10275
Peiyu Qiu et al. (2017) Curcumin Attenuates N-Nitrosodiethylamine-Induced Liver Injury in Mice by Utilizing the Method of Metabonomics. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.6b04797
Zhongyuan Su et al. (2010) Early homing behavior of Stro-1− mesenchyme-like cells derived from human embryonic stem cells in an immunocompetent xenogeneic animal model. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2010.03.033
Jing Liu et al. (2016) Inhibition of diethylnitrosamine-induced liver cancer in rats by Rhizoma paridis saponin. Environmental Toxicology and Pharmacology. 10.1016/j.etap.2016.07.004
Xue-Gong Qin et al. (2010) Effects of matrine on HepG2 cell proliferation and expression of tumor relevant proteins in vitro. Pharmaceutical Biology. 10.3109/13880200903104101
Xiao-Gang Zhong et al. (2007) Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. World Journal of Gastroenterology. 10.3748/wjg.v13.i28.3886
