| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;AFP;HPAFP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AFP (Alpha-fetoprotein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-alpha 1 Fetoprotein/AFP Antibody Picoband® catalog # PB9090. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively. (reported region: Q378-V609).
- Molecular weight context: reported MW: 69 kDa; calculated MW: 68678 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Alpha-fetoprotein; Alpha-fetoprotein. AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours. Functional note: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Reported localization: Secreted. Expression/tissue context: Plasma. Synthesized by the fetal liver and yolk sac.
Research relevance and current trends
- Cancer: Researchers commonly examine how AFP (Alpha-fetoprotein) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how AFP (Alpha-fetoprotein) relates to this theme using model systems and orthogonal readouts.
- Developmental Biology: Researchers commonly examine how AFP (Alpha-fetoprotein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AFP (Alpha-fetoprotein) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of AFP (Alpha-fetoprotein) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the ALB/AFP/VDB family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
