| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein Actn2 ;RCG30552 ;Actn2 ;rCG_30552 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Rabbit skeletal alpha-actinin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) is an antibody targeting ACTN2. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: EA-53; isotype: Mouse IgG1; reactivity: Human,Mouse,Rabbit,Rat; observed MW: 103 kDa; calculated MW: 103834 MW.
Boster Bio Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) catalog # MA1104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Key elements and design rationale
- Target: ACTN2 — actinin, alpha 2
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: EA-53; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rabbit,Rat
- Molecular weight guidance: Observed: 103 kDa; Calculated: 103834 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): ACTN2: F-actin cross-linking protein which is thought to anchoractin to a variety of intracellular structures. This is a bundlingprotein.
Scientific background (datasheet): Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
Cellular localization (datasheet): ACTN2: Cytoplasm, myofibril, sarcomere, Z line|Actn2: Cytoplasm, myofibril, sarcomere, Z line
Tissue details (datasheet): ACTN2: Expressed in both skeletal and cardiac muscle.
Sequence similarities (datasheet): Belongs to the alpha-actinin family.
Research relevance and current trends
- Commonly studied in contexts related to Actin Binding Proteins,Actin, etc.,Cardiovascular,Contractility,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
