| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein Actn2 ;RCG30552 ;Actn2 ;rCG_30552 ; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Rabbit skeletal alpha-actinin. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EA-53 is a mouse monoclonal antibody that recognizes F-actin cross-linking protein (gene Actn2) in human, mouse, rabbit and rat samples and is validated for Western blot and IHC.
Alpha-actinin-2 (ACTN2) is the main alpha-actinin of cardiac and skeletal muscle. It cross-links actin filaments at the Z-disc and anchors the sarcomere, and variants in ACTN2 are associated with cardiomyopathies.
| Target | F-actin cross-linking protein (gene Actn2; UniProt D3ZCV0, rat) |
|---|---|
| Clone | EA-53 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human, Mouse, Rabbit, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | Mouse ascites fluid; 1.2% sodium acetate; 2 mg BSA; with 0.01 mg NaN3 as preservative |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 1–2 µg/mL (Human, Mouse, Rat, Rabbit) |
|---|---|
| Western blot | 0.5–1 µg/mL (Human, Mouse, Rat, Rabbit) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Rabbit skeletal alpha-actinin.
Reactivity Notes
The supplier lists reactivity with human, mouse, rabbit and rat. UniProt places F-actin cross-linking protein in the cell junction and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cordero Julio et al. (2024) Leveraging chromatin state transitions for the identification of regulatory networks orchestrating heart regeneration. Nucleic Acids Research. 10.1093/nar/gkae085
Manna Wang et al. (2020) Mechanical Ring Interfaces between Adherens Junction and Contractile Actomyosin to Coordinate Entotic Cell-in-Cell Formation. Cell Reports. 10.1016/j.celrep.2020.108071
Wen Yuan et al. (2022) Intracellular TMEM16A is necessary for myogenesis of skeletal muscle. iScience. 10.1016/j.isci.2022.105446
Wenjing Liao et al. (2025) Assembly of ceria-Nrf2 nanoparticles as macrophage-targeting ROS scavengers protects against myocardial infarction. Frontiers in Pharmacology. 10.3389/fphar.2024.1503757
Yi Xu et al. (2025) Inhibition of Bif-1 confers cardio-protection in myocardial infarction. American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00473.2024
Fan Jiahui et al. (2019) Nuclear miR-665 aggravates heart failure via suppressing phosphatase and tensin homolog transcription. Science China-Life Sciences. 10.1007/s11427-018-9515-1
Ting-Ting Xiao et al. (2014) Similar to Spironolactone, Oxymatrine Is Protective in Aldosterone-Induced Cardiomyocyte Injury via Inhibition of Calpain and Apoptosis-Inducing Factor Signaling. PLoS One. 10.1371/journal.pone.0088856
Jiaxin Tian et al. (2017) Optimization and enrichment of induced cardiomyocytes derived from mouse fibroblasts by reprogramming with cardiac transcription factors. Molecular Medicine Reports. 10.3892/mmr.2017.8285
Ricardo H. Pires et al. (2022) Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation. Physiological Reports. 10.14814/phy2.15171
