| Field | Specification |
|---|---|
| Target | |
| Alternative names | Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; AR; DHTR; NR3C4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Androgen Receptor/AR recombinant protein (Position: A629-Q920). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects androgen receptor (gene AR) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 99.2 kDa, and the supplier reports an observed band at about 120 kDa.
The androgen receptor (AR) is a ligand-activated nuclear receptor that binds testosterone and dihydrotestosterone. After hormone binding it moves to the nucleus and regulates target genes controlling male sexual development and many other tissues, and it is a central target in prostate cancer research.
| Target | Androgen receptor (gene AR; UniProt P10275, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 99.2 kDa |
| Observed MW | 120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human U2OS cells, human HEK293 cells, human PC-3 cells, human 22RV1 cells, human HepG2 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): T47D cells.
Samples with a confirmed signal (flow cytometry): A549 cells, C6 cells, RAW264.7 cells.
Immunogen
Recombinant human Androgen Receptor/AR fragment (Ala629–Gln920), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places androgen receptor in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Mainly expressed in heart and skeletal muscle... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yifei Cheng et al. (2026) A conserved eIF1A+ luminal cell-centered hypoxic and “cold” tumor microenvironment promotes pan-subtype prostate cancer progression. Cell Reports Medicine. 10.1016/j.xcrm.2026.102619
Miao Jinhua et al. (2023) Sirtuin 6 is a key contributor to gender differences in acute kidney injury. Cell Death Discovery. 10.1038/s41420-023-01432-y
Huang Dong-Yan et al. (2018) Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial–mesenchymal transition in aged rats. Scientific Reports. 10.1038/s41598-017-18869-8
Xiang Cai et al. (2024) JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway. Molecular Carcinogenesis. 10.1002/mc.23753
Ju Guo et al. (2018) Androgen Receptor Is Inactivated and Degraded in Bladder Cancer Cells by Phenyl Glucosamine via miR-449a Restoration. Medical Science Monitor. 10.12659/MSM.906836
Yang Xiao et al. (2018) Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry. Zoological Studies. 10.6620/ZS.2018.57-41
Yan Zhang et al. (2014) Elevated expression levels of androgen receptors and matrix metalloproteinase-2 and -9 in 30 cases of hepatocellular carcinoma compared with adjacent tissues as predictors of cancer invasion and staging. Experimental and Therapeutic Medicine. 10.3892/etm.2014.2150
Qing-Hua Peng et al. (2010) Effects of extract of Buddleja officinalis eye drops on androgen receptors of lacrimal gland cells of castrated rats with dry eye. International Journal of Ophthalmology. 10.3980/j.issn.2222-3959.2010.01.10
