| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-2; ANG-2; ANGPT2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Angiopoietin-2/ANGPT2 recombinant protein (Position: N357-K404). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes angiopoietin-2 (gene ANGPT2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 56.9 kDa, and the supplier reports an observed band at about 66 kDa.
Angiopoietin-2 (ANGPT2) is a TIE2 (TEK) ligand that competes with angiopoietin-1 and often acts as a context-dependent antagonist. It loosens vessel stability, promoting sprouting when VEGF is present and vessel regression when it is not, and it is widely studied in tumor angiogenesis, inflammation and vascular leakage.
| Target | Angiopoietin-2 (gene ANGPT2; UniProt O15123, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 56.9 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SW620 cells, human A549 cells, human K562 cells, rat brain tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue, human placenta tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): HeLa cells, U937 cells.
Immunogen
Recombinant human Angiopoietin-2/ANGPT2 fragment (Asn357–Lys404), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes angiopoietin-2 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Guo et al. (2024) Single-cell analysis reveals the implication of vascular endothelial cell-intrinsic ANGPT2 in human intracranial aneurysm. Cardiovascular Research. 10.1093/cvr/cvae186
Xiaohui Wang et al. (2021) An oxidation responsive nano-radiosensitizer increases radiotherapy efficacy by remolding tumor vasculature. Biomaterials Science. 10.1039/D1BM00834J
Xiaohui Wang et al. (2021) Construction of an AuHQ nano-sensitizer for enhanced radiotherapy efficacy through remolding tumor vasculature. Journal of Materials Chemistry B. 10.1039/D1TB00515D
Shuai B. et al. (2014) Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients. Osteoporosis International. 10.1007/s00198-014-2992-y
Xiaoyan Bai et al. (2014) Antiangiogenic Treatment Diminishes Renal Injury and Dysfunction via Regulation of Local AKT in Early Experimental Diabetes. PLoS One. 10.1371/journal.pone.0096117
Lijun Xu et al. (2025) Comprehensive analysis and validation of angiogenesis in vascular dementia from the perspective of diagnosis, prevention, and treatment. Frontiers in Genetics. 10.3389/fgene.2025.1646991
Li Hao et al. (2014) The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery. Cardiovascular Pathology. 10.1016/j.carpath.2014.03.009
Xiang Li et al. (2016) Expression of angiopoietins in central nervous system hemangioblastomas is associated with cyst formation. Neuroscience Letters. 10.1016/j.neulet.2016.12.066
Qingping Zheng et al. (2011) Exercise Improves Recovery after Ischemic Brain Injury by Inducing the Expression of Angiopoietin-1 and Tie-2 in Rats. Tohoku Journal of Experimental Medicine. 10.1620/tjem.224.221
Cai Jingting et al. (2006) Expression of heparanase and angiopoietin-2 in patients with endometriosis. European Journal of Obstetrics & Gynecology and Reproductive Biology. 10.1016/j.ejogrb.2006.09.018
