| Field | Specification |
|---|---|
| Target | |
| Alternative names | Type-1 angiotensin II receptor;AT1AR;AT1BR;Angiotensin II type-1 receptor;AT1;AGTR1;AGTR1A, AGTR1B, AT2R1, AT2R1B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human AGTR1, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody detects type-1 angiotensin II receptor (gene AGTR1) in human, monkey, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 41.1 kDa, and the supplier reports an observed band at about 50 kDa.
The angiotensin II type 1 receptor (AT1R, AGTR1) is a G protein-coupled receptor that mediates most known actions of angiotensin II. Through Gq and phospholipase C it raises intracellular Ca2+ and drives vasoconstriction, aldosterone release and sodium retention, and it is the target of angiotensin receptor blocker drugs used in hypertension research.
| Target | Type-1 angiotensin II receptor (gene AGTR1; UniProt P30556, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 41.1 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
Samples with a confirmed band (WB): human HEK293 cells, human T-47D cells, human PC-3 cells, human U-87MG cells, human HepG2 cells, monkey COS-7 cells, rat brain tissue, rat liver tissue, rat kidney tissue, rat RH35 cells, mouse brain tissue, mouse liver tissue, mouse kidney tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human AGTR1. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places type-1 angiotensin II receptor in the cell membrane. Tissue expression noted by UniProt (human): Liver, lung, adrenal and adrenocortical adenomas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qinyu Wu et al. (2023) The CXCR4-AT1 axis plays a vital role in glomerular injury via mediating the crosstalk between podocyte and mesangial cell. Translational Research. 10.1016/j.trsl.2023.09.005
Gomes Karina Pereira et al. (2020) Antiepileptic effects of long-term intracerebroventricular infusion of angiotensin-(1-7) in an animal model of temporal lobe epilepsy. Clinical Science. 10.1042/CS20200514
Beatriz Martín-Carro et al. (2025) Expression of the Renin-Angiotensin System in the Heart, Aorta, and Perivascular Adipose Tissue in an Animal Model of Type 1 Diabetes. International Journal of Molecular Sciences. 10.3390/ijms26199538
Shuai B. et al. (2014) Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients. Osteoporosis International. 10.1007/s00198-014-2992-y
Hanxing Liu et al. (2022) Network Pharmacology and Molecular Docking-Based Mechanism Study to Reveal Antihypertensive Effect of Gedan Jiangya Decoction. Biomed Research International. 10.1155/2022/3353464
Zhiyong Wu et al. (2016) Angiotensin II induces apoptosis of human pulmonary microvascular endothelial cells in acute aortic dissection complicated with lung injury patients through modulating the expression of monocyte chemoattractant protein-1. American Journal of Translational Research. PMID: 27069537
Jian Jia et al. (2022) Vitamin D receptor deficiency increases systolic blood pressure by upregulating the renin‑angiotensin system and autophagy. Experimental and Therapeutic Medicine. 10.3892/etm.2022.11243
Wen-Bin Liu et al. (2005) Effects of angiotensin II receptor antagonist, Losartan on the apoptosis, proliferation and migration of the human pancreatic stellate cells. World Journal of Gastroenterology. 10.3748/wjg.v11.i41.6489
