| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Angiotensinogen;Serpin A8;Angiotensin-1;Angiotensin 1-10;Angiotensin I;Ang I;Angiotensin-2;Angiotensin 1-8;Angiotensin II;Ang II;Angiotensin-3;Angiotensin 2-8;Angiotensin III;Ang III;Des-Asp[1]-angiotensin II;Angiotensin-4;Angiotensin 3-8;Angiotensin IV;Ang IV;Angiotensin 1-9;Angiotensin 1-7;Angiotensin 1-5;Angiotensin 1-4;Agt;Serpina8; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse Angiotensinogen recombinant protein (Position: D25-A115). Mouse Angiotensinogen shares 62.2% and 87.9% amino acid (aa) sequence identity with human and rat Angiotensinogen, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Agt (Angiotensinogen) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Angiotensinogen/AGT Antibody Picoband® catalog # A02103-1. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived mouse Angiotensinogen recombinant protein (Position: D25-A115). Mouse Angiotensinogen shares 62.2% and 87.9% amino acid (aa) sequence identity with human and rat Angiotensinogen, respectively. (reported region: D25-A115).
- Molecular weight context: reported MW: 53-55 kDa; calculated MW: 51990 MW
- Reactivity: Mouse
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Angiotensinogen; Angiotensinogen. AGT (ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. Functional note: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Reported localization: Secreted. Expression/tissue context: Expressed by the liver and secreted in plasma.
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how Agt (Angiotensinogen) relates to this theme using model systems and orthogonal readouts.
- G Protein Signaling: Researchers commonly examine how Agt (Angiotensinogen) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how Agt (Angiotensinogen) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Agt (Angiotensinogen) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Agt (Angiotensinogen) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
