| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Annexin A5;Anchorin CII;Annexin V;Annexin-5;Calphobindin I;CBP-I;Endonexin II;Lipocortin V;Placental anticoagulant protein 4;PP4;Placental anticoagulant protein I;PAP-I;Thromboplastin inhibitor;Vascular anticoagulant-alpha;VAC-alpha;ANXA5;ANX5, ENX2, PP4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Annexin V |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ANXA5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAAB-1; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Annexin V ANXA5 Rabbit Monoclonal Antibody catalog # M00744. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ANXA5 (Annexin A5).
- Antibody format: Monoclonal; clone AAAB-1; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ANXA5 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): This protein is an anticoagulant protein that acts as an in inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Reported cellular localization context: Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane ; Peripheral membrane protein . Nucleus. Tissue expression notes (as provided): Ubiquitous. Expressed at high levels in testis.
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Apoptotic Markers,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cell Death,Invasion/Microenvironment.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate ANXA5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ANXA5 expression by Western blot in cell or tissue lysates, Localize ANXA5 by immunofluorescence/immunocytochemistry in cultured cells, Quantify ANXA5-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 133 kDa; calculated MW: 35937 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 133 kDa
- Cellular localization (provided): Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane ; Peripheral membrane protein . Nucleus.
- Tissue details (provided): Ubiquitous. Expressed at high levels in testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
