| Field | Specification |
|---|---|
| Target | |
| Alternative names | Natriuretic peptides A; Prepronatriodilatin; Atrial natriuretic factor; ANF; Atrial natriuretic peptide; ANP; Auriculin-B; Auriculin-A; Atriopeptin-1; Atriopeptin I; Atriopeptin-2; Atriopeptin II; Atriopeptin-3; Atriopeptin III; Nppa |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat ANP recombinant protein (Position: N25-R122). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes natriuretic peptides A (gene Nppa) in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 16.6 kDa, and the supplier reports an observed band at about 19 kDa.
Atrial natriuretic peptide (ANP), encoded by NPPA, is a hormone released mainly by atrial cardiomyocytes in response to stretch. It binds the receptor NPR1 to raise cGMP, causing vasodilation, sodium and water excretion and lower blood pressure. ANP is studied in cardiovascular and renal homeostasis and as a marker of cardiac stress.
| Target | Natriuretic peptides A (gene Nppa; UniProt P01161, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 16.6 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat cardiac muscle tissue, mouse cardiac muscle tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant rat ANP fragment (Asn25–Arg122), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. Tissue expression noted by UniProt (rat): High levels of expression in the atria compared to the ventricles. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xingpeng Bu et al. (2026) Thrombospondin 1 Aggravates Cardiac Remodeling in Heart Failure with Preserved Ejection Fraction by Inhibiting Mitophagy. iScience. 10.1016/j.isci.2026.114639
Wang Kang et al. (2024) Actn2 defects accelerates H9c2 hypertrophy via ERK phosphorylation under chronic stress. Genes & Genomics. 10.1007/s13258-024-01536-4
