| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aquaporin-11;AQP-11;AQP11;AQPX1;PSEC0027; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human AQP11, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes aquaporin-11 (gene AQP11) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 30.2 kDa, and the supplier reports an observed band at about 30 kDa.
Aquaporin-11 (AQP11) is an unconventional aquaporin located in intracellular membranes, mainly the endoplasmic reticulum. It can transport water, glycerol and hydrogen peroxide, and it is studied for its contribution to ER homeostasis, particularly in kidney proximal tubule cells.
| Target | Aquaporin-11 (gene AQP11; UniProt Q8NBQ7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 30.2 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human AQP11. It differs from the mouse sequence at two residues and from the rat sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aquaporin-11 in the endoplasmic reticulum membrane, cytoplasmic vesicle membrane and cell membrane. Tissue expression noted by UniProt (human): Detected in the sperm head and tail (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Giorgia Pellavio et al. (2025) Sigma1 Receptor Modulates Plasma Membrane and Mitochondrial Peroxiporins. Cells. 10.3390/cells14141082
