| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Lens fiber major intrinsic protein;Aquaporin-0;MIP26;MP26;MIP;AQP0; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 0, different from the related mouse and rat sequences by six amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MIP (Lens fiber major intrinsic protein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Aquaporin 0/MIP Antibody Picoband® catalog # PB9811. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 0, different from the related mouse and rat sequences by six amino acids.
- Molecular weight context: reported MW: 28 kDa; calculated MW: 28122 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Lens fiber major intrinsic protein; Lens fiber major intrinsic protein. Lens fiber major intrinsic protein, also called MIP26 or MP26, is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core. Functional note: Water channel (PubMed:24120416). Channel activity is down-regulated by CALM when cytoplasmic Ca (2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core (By similarity). Plays a role in cell-to-cell adhesion and facilitates gap junction coupling (PubMed:24120416). . Reported localization: Cell membrane ; Multi- pass membrane protein . Cell junction, gap junction . Expression/tissue context: Major component of lens fiber gap junctions.
Research relevance and current trends
- Immunology: Researchers commonly examine how MIP (Lens fiber major intrinsic protein) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how MIP (Lens fiber major intrinsic protein) relates to this theme using model systems and orthogonal readouts.
- Macrophage/Inflammation: Researchers commonly examine how MIP (Lens fiber major intrinsic protein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MIP (Lens fiber major intrinsic protein) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
