| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lens fiber major intrinsic protein;Aquaporin-0;MIP26;MP26;MIP;AQP0; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 0, different from the related mouse and rat sequences by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets lens fiber major intrinsic protein (gene MIP) in human and rat samples and is validated for Western blot. The predicted molecular weight is 28.1 kDa, and the supplier reports an observed band at about 28 kDa.
Aquaporin-0 (AQP0, encoded by MIP) is the most abundant membrane protein of the eye lens fiber cells. It forms water channels and also acts as an adhesion molecule linking neighboring fiber cells, both of which help keep the lens transparent, and mutations in MIP are a known cause of inherited cataract.
| Target | Lens fiber major intrinsic protein (gene MIP; UniProt P30301, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 28.1 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
|---|
Samples with a confirmed band (WB): rat eye ball tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human aquaporin 0. It differs from the mouse and rat sequences at six residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places lens fiber major intrinsic protein in the cell membrane and cell junction. Tissue expression noted by UniProt (human): Expressed in the cortex and nucleus of the retina lens (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
