| Field | Specification |
|---|---|
| Target | |
| Alternative names | Activity-regulated cytoskeleton-associated protein;ARC/ARG3.1;Activity-regulated gene 3.1 protein homolog;Arg3.1;ARC ;KIAA0278 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Arc, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes activity-regulated cytoskeleton-associated protein (gene ARC) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 45.3 kDa, and the supplier reports an observed band at about 45 kDa.
Arc (activity-regulated cytoskeleton-associated protein) is an immediate early gene product induced rapidly by neuronal activity. It regulates AMPA receptor endocytosis and is required for long-term synaptic plasticity and memory consolidation, and it can form virus-like capsids that transfer RNA between neurons.
| Target | Activity-regulated cytoskeleton-associated protein (gene ARC; UniProt Q7LC44, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 45.3 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human U2OS cells, human 293T cells, human SH-SY5Y cells, human SiHa cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human arc. It differs from the mouse sequence at two residues and from the rat sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places activity-regulated cytoskeleton-associated protein in the extracellular vesicle membrane, postsynaptic cell membrane, synapse and postsynaptic density. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Yue et al. (2009) Anti-apoptotic Effects of a Calpain Inhibitor on Cardiomyocytes in a Canine Rapid Atrial Fibrillation Model. Cardiovascular Drugs and Therapy. 10.1007/s10557-009-6199-y
