| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aromatase;1.14.14.14;CYPXIX;Cytochrome P-450AROM;Cytochrome P450 19A1;Estrogen synthase;CYP19A1;ARO1, CYAR, CYP19; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Aromatase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AABB-3 is a rabbit monoclonal antibody that recognizes aromatase (gene CYP19A1) in human and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 57.9 kDa, and the supplier reports an observed band at about 50 kDa.
Aromatase (CYP19A1) is the cytochrome P450 enzyme that converts androgens into estrogens, turning androstenedione into estrone and testosterone into estradiol. It is expressed in the ovary, placenta, adipose tissue, bone and brain, and it is the target of aromatase inhibitors used in breast cancer, so it is central to studies of estrogen biology and hormone-dependent cancers.
| Target | Aromatase (gene CYP19A1; UniProt P11511, human) |
|---|---|
| Clone | AABB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.9 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human A549 cells, human Caco-2 cells, human 293T cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human aromatase.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places aromatase in the endoplasmic reticulum membrane and microsome membrane. Tissue expression noted by UniProt (human): Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hai-Tao Gao et al. (2020) Zinc supplement ameliorates phthalates-induced reproductive toxicity in male rats. Chemosphere. 10.1016/j.chemosphere.2020.125828
