| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mimecan; Osteoglycin; Ogn; Og |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Astrin/Deepest/SPAG5 recombinant protein (Position: E738-S1193). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Astrin/Deepest/SPAG5 Antibody Picoband® is an antibody for SPAG5 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SPAG5 (osteoglycin); UniProt: Q96R06
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 130 kDa, 150 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Astrin/Deepest/SPAG5 Antibody Picoband® catalog # A07062-2.
Biological background
Biological context: Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
Expression and localization notes: cellular localization: Secreted, extracellular space, extracellular matrix., tissue context: Expressed in kidney..
Common research applications
- Western blotting (WB): Compare SPAG5 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SPAG5 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify SPAG5-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Sperm-associated antigen 5 is a protein that in humans is encoded by the SPAG5 gene. This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted, extracellular space, extracellular matrix.
- Tissue details: Expressed in kidney.
- Research category: Cell Biology,Cell Cycle,Cell Differentiation,Cytoskeleton/ECM,Extracellular Matrix,Signal Transduction,Structures
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
