| Field | Specification |
|---|---|
| Target | |
| Alternative names | Autophagy protein 5;APG5-like;Apoptosis-specific protein;ATG5;APG5L, ASP; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATG5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GCD-1 is a rabbit monoclonal antibody that recognizes autophagy protein 5 (gene ATG5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 32.4 kDa, and the supplier reports an observed band at about 55 kDa.
ATG5 is a core autophagy protein that is covalently linked to ATG12 by the enzymes ATG7 and ATG10. The ATG12-ATG5 conjugate, bound to ATG16L1, drives LC3 lipidation and autophagosome formation, and ATG5 knockout cells and animals are standard tools for studying autophagy.
| Target | Autophagy protein 5 (gene ATG5; UniProt Q9H1Y0, human) |
|---|---|
| Clone | GCD-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.4 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human MCF-7 cells, human K562 cells, rat liver tissue, rat C6 cells, mouse liver tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human ovarian cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): Raji cells.
Immunogen
Synthetic peptide based on the sequence of human ATG5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places autophagy protein 5 in the cytoplasm and preautophagosomal structure membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tingting Wang et al. (2022) Synergistic Lysosomal Impairment and ER Stress Activation for Boosted Autophagy Dysfunction Based on Te Double-Headed Nano-Bullets. Small. 10.1002/smll.202201585
Chao Zhong et al. (2025) R406 and its structural analogs reduce SNCA/α-synuclein levels via autophagic degradation. Autophagy. 10.1080/15548627.2025.2483886
Ying Zhu et al. (2025) Momordica Charantia L. polysaccharides ameliorate colonic mucus barrier damage induced by high fat diet through regulation of gut microbiota and MUC2 expression. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.148065
Li Tingting et al. (2024) NCX1/Ca2+ promotes autophagy and decreases bortezomib activity in multiple myeloma through non-canonical NFκB signaling pathway. Cell Communication and Signaling. 10.1186/s12964-024-01628-4
Bei Chen et al. (2023) 1,3-Substituted β-Carboline Derivatives as Potent Chemotherapy for the Treatment of Cystic Echinococcosis. Journal of Medicinal Chemistry. 10.1021/acs.jmedchem.3c01326
Zhiqiang Feng et al. (2024) Liensinine sensitizes colorectal cancer cells to oxaliplatin by targeting HIF-1a to inhibit autophagy. Phytomedicine. 10.1016/j.phymed.2024.155647
Xiaochun Huang et al. (2025) TRPV1 mediates oxidative stress and autophagy pathways to regulate airway MUC5AC secretion. Biochemical Pharmacology. 10.1016/j.bcp.2025.117658
Ya Chen et al. (2022) eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish. Journal of Biological Chemistry. 10.1016/j.jbc.2022.101730
Jing-jing Xing et al. (2019) Ginsenoside Rb3 provides protective effects against cisplatin-induced nephrotoxicity via regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo. Cell Proliferation. 10.1111/cpr.12627
Ji-Xian Song et al. (2025) Hydrogen alleviates cardiac ferroptosis damage under chronic intermittent hypoxia conditions by targeting Nrf2. International Immunopharmacology. 10.1016/j.intimp.2025.115974
