| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death 1 ligand 2;PD-1 ligand 2;PD-L2;PDCD1 ligand 2;Programmed death ligand 2;Butyrophilin B7-DC;B7-DC;CD273;PDCD1LG2;B7DC, CD273, PDCD1L2, PDL2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human B7 DC recombinant protein (Position: L20-T220). Human B7 DC shares 72.1% amino acid (aa) sequence identity with mouse B7 DC. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets programmed cell death 1 ligand 2 (gene PDCD1LG2) in human samples and is validated for Western blot. The predicted molecular weight is 31.0 kDa, and the supplier reports an observed band at about 37 kDa.
PD-L2 (PDCD1LG2, CD273) is a ligand for the inhibitory receptor PD-1. It is expressed mainly on dendritic cells and macrophages and, by engaging PD-1, reduces T-cell proliferation and cytokine production, helping to maintain immune tolerance.
| Target | Programmed cell death 1 ligand 2 (gene PDCD1LG2; UniProt Q9BQ51, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 31.0 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human B7 DC fragment (Leu20–Thr220), expressed in E. coli. Sequence identity with the mouse ortholog: 72.1%.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
