| Field | Specification |
|---|---|
| Target | |
| Alternative names | BAG family molecular chaperone regulator 1;BAG-1;Bcl-2-associated athanogene 1;BAG1;HAP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BAG1, which shares 91.4% and 88.6% amino acid (aa) sequence identity with mouse and rat BAG1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes BAG family molecular chaperone regulator 1 (gene BAG1) in human samples and is validated for Western blot. The predicted molecular weight is 38.8 kDa; the supplier reports observed bands at 33 kDa(S), 46 kDa(M), 56 kDa(L).
BAG1 is a co-chaperone that binds HSP70 and HSC70 through its BAG domain and acts as a nucleotide exchange factor, promoting ADP release and substrate unloading. It also contains a ubiquitin-like domain that links chaperone clients to the proteasome, and it is studied in protein quality control, apoptosis and steroid receptor signaling.
| Target | BAG family molecular chaperone regulator 1 (gene BAG1; UniProt Q99933, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 38.8 kDa |
| Observed MW | 33 kDa(S), 46 kDa(M), 56 kDa(L) |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human BAG1. Sequence identity with the mouse and rat orthologs: 91.4% and 88.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Isoform 4 is the most abundantly expressed isoform. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
