| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BMP and activin membrane-bound inhibitor homolog; Non-metastatic gene A protein; Putative transmembrane protein; NMA; BAMBI; NMA |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human BAMBI/NMA recombinant protein (Position: K25-V260). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-BAMBI/NMA Picoband® Antibody is an antibody for BAMBI detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: BAMBI (BMP and activin membrane bound inhibitor); UniProt: Q13145; NCBI Gene: 25805
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 29 kDa, calculated 17136 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-BAMBI/NMA Picoband® Antibody catalog # A07983-1.
Biological background
Biological context: Negatively regulates TGF-beta signaling.
Expression and localization notes: cellular localization: Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock., tissue context: Expressed in adult liver..
Common research applications
- Western blotting (WB): Compare BAMBI levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of BAMBI in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify BAMBI-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: BMP and activin membrane-bound inhibitor homolog (Xenopus laevis), also known as BAMBI, is a protein which in humans is encoded by the BAMBI gene. It is mapped to 10p12.1. This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.
- Tissue details: Expressed in adult liver.
- Research category: Atherosclerosis,Cardiovascular,Chaperones,Heat Shock Proteins,Protein Trafficking,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
