| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C motif chemokine 13;B lymphocyte chemoattractant;CXC chemokine BLC;Small-inducible cytokine B13;Cxcl13;Blc, Scyb13; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes C-X-C motif chemokine 13 (gene Cxcl13) in mouse samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 11.9 kDa, and the supplier reports an observed band at about 11 kDa.
CXCL13 (BLC) is a chemokine that attracts B cells through the receptor CXCR5. Produced by stromal cells in lymphoid follicles, it organizes B-cell follicles and guides follicular helper T cells. CXCL13 is also studied in ectopic lymphoid structures that form in chronic inflammation, autoimmunity and some tumors.
| Target | C-X-C motif chemokine 13 (gene Cxcl13; UniProt O55038, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 11.9 kDa |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): recombinant mouse BCA1/CXCL13 protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse BCA1 fragment (Ile22–Ala109), expressed in E. coli. Sequence identity with the human ortholog: 45.3%.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes C-X-C motif chemokine 13 as a secreted protein. Tissue expression noted by UniProt (mouse): Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Masahiro Maeyama et al. (2020) Multi-marker algorithms based on CXCL13, IL-10, sIL-2 receptor, and β2-microglobulin in cerebrospinal fluid to diagnose CNS lymphoma. Cancer Medicine. 10.1002/cam4.3048
