| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-X-C motif chemokine 13;B lymphocyte chemoattractant;CXC chemokine BLC;Small-inducible cytokine B13;Cxcl13;Blc, Scyb13; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Cxcl13 (C-X-C motif chemokine 13) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-BCA1/CXCL13 Antibody Picoband® catalog # PB9999. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1. (reported region: I22-A109).
- Molecular weight context: reported MW: 11 kDa; calculated MW: 12 kDa
- Reactivity: Mouse
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-X-C motif chemokine 13; C-X-C motif chemokine 13. C-X-C motif chemokine 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells. Functional note: Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in ing the migration of B-lymphocytes to follicles in secondary lymphoid organs. Reported localization: Secreted. Expression/tissue context: Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles).
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how Cxcl13 (C-X-C motif chemokine 13) relates to this theme using model systems and orthogonal readouts.
- Heart Disease: Researchers commonly examine how Cxcl13 (C-X-C motif chemokine 13) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how Cxcl13 (C-X-C motif chemokine 13) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Cxcl13 (C-X-C motif chemokine 13) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Cxcl13 (C-X-C motif chemokine 13) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
