| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interferon regulatory factor 5;IRF-5;IRF5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human BCAR1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-BCAR1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23B56; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-BCAR1 Rabbit Monoclonal Antibody catalog # M00960-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: BCAR1 (Interferon regulatory factor 5).
- Antibody format: Monoclonal; clone 23B56; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
BCAR1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. . Reported cellular localization context: Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Tissue expression notes (as provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Research relevance and current trends
- Research context keywords from the source record include: Cytokines,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Immunology,Innate Immunity,Interferons,Polymerase Associated Factors,Transcription,Transcription Factors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate BCAR1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect BCAR1 expression by Western blot in cell or tissue lysates, Compare relative BCAR1 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 130 kDa; calculated MW: 56044 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 130 kDa
- Cellular localization (provided): Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Tissue details (provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
