| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptosis regulator Bcl-2;Bcl2;Bcl-2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone Bcl-2-100 is a mouse monoclonal antibody that recognizes apoptosis regulator Bcl-2 (gene Bcl2) in human samples and is validated for Western blot, IHC and ICC/IF.
Bcl-2 (BCL2) is an anti-apoptotic protein located mainly on the mitochondrial outer membrane that binds and restrains pro-apoptotic Bcl-2 family members such as BAX, BAK and BH3-only proteins. It was first identified through the t(14;18) translocation in follicular lymphoma, and it is a central regulator of mitochondrial apoptosis and a target of BH3-mimetic drugs.
| Target | Apoptosis regulator Bcl-2 (gene Bcl2; UniProt P49950, rat) |
|---|---|
| Clone | Bcl-2-100 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.4–1 µg/mL (Human) |
|---|---|
| Western blot | 1–2 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Immunogen
Synthetic peptide based on the sequence of corresponding to residues 41–54 of the bcl-2 protein, conjugated to thyroglobulin.
Reactivity Notes
The supplier lists reactivity with human. UniProt places apoptosis regulator Bcl-2 in the mitochondrion outer membrane, nuclear membrane, endoplasmic reticulum membrane and cytoplasm. Tissue expression noted by UniProt (rat): Expressed in a variety of tissues, with highest levels in reproductive tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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