| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptosis regulator Bcl-2;BCL2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Bcl-2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HF-2 is a rabbit monoclonal antibody that recognizes apoptosis regulator Bcl-2 (gene BCL2) in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 26.3 kDa, and the supplier reports an observed band at about 26 kDa.
Bcl-2 (BCL2) is an anti-apoptotic protein located mainly on the mitochondrial outer membrane that binds and restrains pro-apoptotic Bcl-2 family members such as BAX, BAK and BH3-only proteins. It was first identified through the t(14;18) translocation in follicular lymphoma, and it is a central regulator of mitochondrial apoptosis and a target of BH3-mimetic drugs.
| Target | Apoptosis regulator Bcl-2 (gene BCL2; UniProt P10415, human) |
|---|---|
| Clone | HF-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.3 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:100 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:100 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): human non-Hodgkin's lymphoma tissue, human squamous cells carcinoma tissue, rat liver tissue, mouse skin tissue, rat stomach tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): Jurkat cells.
Immunogen
Synthetic peptide based on the sequence of human bcl-2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places apoptosis regulator Bcl-2 in the mitochondrion outer membrane, nuclear membrane, endoplasmic reticulum membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huijuan Zeng et al. (2018) Downregulation of long non-coding RNA Opa interacting protein 5-antisense RNA 1 inhibits breast cancer progression by targeting sex-determining region Y-box 2 by microRNA-129-5p upregulation. Cancer Science. 10.1111/cas.13879
Huijuan Zeng et al. (2018) microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2. Archives of Biochemistry and Biophysics. 10.1016/j.abb.2018.05.018
