| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | HRP-Actin, Beta-Actin, Actin, Beta actin, b actin |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Gene ID | |
| Host | |
| Immunogen | HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-beta-Actin ACTB Antibody Picoband® (HRP) is an antibody for ACTB detection raised in Rabbit (Polyclonal, IgG, HRP), with reported reactivity: Chicken,Human,Monkey,Mouse,Rat,Zebrafish. Commonly used in WB workflows.
Key elements and design rationale
- Target: ACTB (Actin, beta); UniProt: P60709; NCBI Gene: 60
- Antibody format: Rabbit, Polyclonal, IgG, HRP
- Molecular weight: 42 kDa, calculated 41737 MW
- Applications: WB
Vendor description (summary): Boster Bio Anti-beta-Actin ACTB Antibody (HRP) (Catalog # A01263-HRP).
Biological background
Biological context: Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G- actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
Expression and localization notes: cellular localization: Cytoplasm, cytoskeleton, tissue context: Widely expressed, with the highest expression in lung, spleen and thymus..
Common research applications
- Western blotting (WB): Compare ACTB levels across samples and conditions using appropriate loading and biological controls.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
- Cross reactivity: HRP-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.
- Cellular localization: Cytoplasm, cytoskeleton
- Tissue details: Widely expressed, with the highest expression in lung, spleen and thymus.
- Sequence similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
- Research category: Biochemicals,Chemical Type
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
