| Field | Specification |
|---|---|
| Target | |
| Alternative names | Actin, cytoplasmic 1; Beta-actin; ACTB |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects actin, cytoplasmic 1 (gene ACTB) in chicken, human, monkey, mouse, rat and zebrafish samples and is validated for Western blot. The predicted molecular weight is 41.7 kDa, and the supplier reports an observed band at about 42 kDa.
Beta-actin (ACTB) is a cytoplasmic actin found in nearly all cells that assembles into filaments forming the cytoskeleton. It supports cell shape, migration and division and also acts in the nucleus in gene regulation, and it is widely used as a loading control in Western blot.
| Target | Actin, cytoplasmic 1 (gene ACTB; UniProt P60709, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Chicken, Human, Monkey, Mouse, Rat, Zebrafish |
| Form | Liquid |
| Formulation | 50% glycerol; 0.9% NaCl; 0.2% Na2HPO4 |
| Calculated MW | 41.7 kDa |
| Observed MW | 42 kDa |
| Storage | Store at 4 °C. |
Validated Applications
| Western blot | 0.02–0.1 µg/mL |
|---|
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human MCF-7 cells, human K562 cells, human A549 cells, human A431 cells, human U87 cells, monkey COS-7 cells, rat heart tissue, rat skeletal muscle tissue, rat brain tissue, rat PC-12 cells, mouse heart tissue, mouse skeletal tissue, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.1 µg/mL overnight at 4 °C, ECL detection.
Immunogen
HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.
Reactivity Notes
The supplier lists reactivity with chicken, human, monkey, mouse, rat and zebrafish. UniProt places actin, cytoplasmic 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
