| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Polycomb complex protein BMI-1;Polycomb group RING finger protein 4;RING finger protein 51;BMI1;PCGF4, RNF51; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human BGAT |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ABO antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24A61; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-BGAT Rabbit Monoclonal Antibody catalog # M00315-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ABO (Polycomb complex protein BMI-1).
- Antibody format: Monoclonal; clone 24A61; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ABO (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. . Reported cellular localization context: Nucleus. Cytoplasm. Tissue expression notes (as provided): Ubiquitously expressed in various human primary cells and tumor cell lines. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Differentiation,2339,Chromatin Remodeling,Epigenetics and Nuclear Signaling,Hematopoietic Progenitors,Oncoproteins,Oncoproteins/Suppressors,Proto-Oncogenes,Stem Cells,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate ABO antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ABO expression by Western blot in cell or tissue lysates, Enrich ABO by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 41 kDa; calculated MW: 36949 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 41 kDa
- Cellular localization (provided): Nucleus. Cytoplasm.
- Tissue details (provided): Ubiquitously expressed in various human primary cells and tumor cell lines. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
