| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Phosphatidylinositol 3-kinase regulatory subunit gamma;PI3-kinase regulatory subunit gamma;PI3K regulatory subunit gamma;PtdIns-3-kinase regulatory subunit gamma;Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma;PI3-kinase subunit p55-gamma;PtdIns-3-kinase regulatory subunit p55-gamma;p55PIK;PIK3R3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human BHMT |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-BHMT antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23B50; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-BHMT Rabbit Monoclonal Antibody catalog # M06712-2. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: BHMT (Phosphatidylinositol 3-kinase regulatory subunit gamma).
- Antibody format: Monoclonal; clone 23B50; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
BHMT (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Immunology,Innate Immunity,Lipid Signaling,Signal Transduction,Signaling Pathway,TLR Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate BHMT antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect BHMT expression by Western blot in cell or tissue lysates, Detect BHMT in FFPE tissue sections by immunohistochemistry, Enrich BHMT by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 45 kDa; calculated MW: 54448 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 45 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
