| Field | Specification |
|---|---|
| Target | |
| Alternative names | Betaine--homocysteine S-methyltransferase 1; BHMT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BHMT |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23B50 is a rabbit monoclonal antibody that recognizes betaine--homocysteine S-methyltransferase 1 (gene BHMT) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 45.0 kDa, and the supplier reports an observed band at about 45 kDa.
Betaine–homocysteine S-methyltransferase (BHMT) is a zinc enzyme highly expressed in liver and kidney that transfers a methyl group from betaine to homocysteine, producing dimethylglycine and methionine. It provides a folate-independent route for homocysteine remethylation and links choline oxidation with methionine metabolism.
| Target | Betaine--homocysteine S-methyltransferase 1 (gene BHMT; UniProt Q93088, human) |
|---|---|
| Clone | 23B50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.0 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BHMT.
Reactivity Notes
The supplier lists reactivity with human. UniProt places betaine--homocysteine S-methyltransferase 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Found exclusively in liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
