| Field | Specification |
|---|---|
| Target | |
| Alternative names | Growth/differentiation factor 11;GDF-11;Bone morphogenetic protein 11;BMP-11;GDF11;BMP11; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BMP11 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOAG-7 is a rabbit monoclonal antibody that recognizes growth/differentiation factor 11 (gene GDF11) in human and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 45.1 kDa, and the supplier reports an observed band at about 45 kDa.
GDF11 (also called BMP-11) is a secreted TGF-β superfamily ligand closely related to myostatin. It signals through activin type II and type I receptors to activate SMAD2/3 and is important for anterior–posterior patterning of the axial skeleton during development. Its effects in aging tissues have been debated and remain an active research area.
| Target | Growth/differentiation factor 11 (gene GDF11; UniProt O95390, human) |
|---|---|
| Clone | AOAG-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.1 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): rat C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human thyroid cancer tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human BMP11.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt describes growth/differentiation factor 11 as a secreted protein. Tissue expression noted by UniProt (human): In the embryo, strong expression is seen in the palatal epithelia, including the medial edge epithelial and midline epithelial seam of the palatal shelves. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yi Zhang et al. (2023) Effect of growth differentiation factor 11 expression after peripheral nerve injury in Sprague-Dawley rats. Neurological Research. 10.1080/01616412.2023.2211446
