| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 15;BMP-15;Growth/differentiation factor 9B;GDF-9B;BMP15;GDF9B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human BMP15 recombinant protein (Position: Q268-R392). Human BMP15 shares 69.6% amino acid (aa) sequence identity with mouse BMP15. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects bone morphogenetic protein 15 (gene BMP15) in human samples and is validated for Western blot. The predicted molecular weight is 45.1 kDa, and the supplier reports an observed band at about 56 kDa.
Bone morphogenetic protein 15 (BMP15) is an oocyte-derived growth factor of the TGF-β superfamily. It acts on surrounding granulosa cells, often together with GDF9, to promote their growth and follicle development, and variants in BMP15 are linked to ovarian insufficiency and differences in ovulation rate in several species.
| Target | Bone morphogenetic protein 15 (gene BMP15; UniProt O95972, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 45.1 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HeLa cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human BMP15 fragment (Gln268–Arg392), expressed in E. coli. Sequence identity with the mouse ortholog: 69.6%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes bone morphogenetic protein 15 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
