| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calcitonin gene-related peptide 1;Alpha-type CGRP;Calcitonin gene-related peptide I;CGRP-I;Calca;Calc; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Calcitonin, different from the related human sequence by four amino acids, and identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects calcitonin gene-related peptide 1 (gene Calca) in mouse and rat samples and is validated for IHC. The predicted molecular weight is 14.1 kDa.
CALCA encodes calcitonin gene-related peptide 1 (αCGRP), produced by alternative splicing of the calcitonin gene in sensory and central neurons. CGRP is a powerful vasodilator acting through the CALCRL–RAMP1 receptor and a key mediator of neurogenic inflammation and pain signaling. It is a major target in migraine research.
| Target | Calcitonin gene-related peptide 1 (gene Calca; UniProt Q99JA0, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 14.1 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of mouse calcitonin. The peptide sequence is conserved in rat. It differs from the human sequence at four residues.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes calcitonin gene-related peptide 1 as a secreted protein. Tissue expression noted by UniProt (mouse): Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bi-Qin Lai et al. (2013) The integration of NSC-derived and host neural networks after rat spinal cord transection. Biomaterials. 10.1016/j.biomaterials.2012.12.046
Xing-Long Gu et al. (2007) The colocalization of CGRP receptor and AMPA receptor in the spinal dorsal horn neuron of rat: A morphological and electrophysiological study. Neuroscience Letters. 10.1016/j.neulet.2006.12.056
Yan Li et al. (2009) Effects of Calcitonin Gene-Related Peptide on the Expression and Activity of Nitric Oxide Synthase During Mandibular Bone Healing in Rabbits: An Experimental Study. Journal of Oral and Maxillofacial Surgery. 10.1016/j.joms.2008.06.077
Hangyu Xu et al. (2018) Effect of chronic hypoxia and hypercapnia on learning and memory function in mice and the expression of NT and CGRP in brain. European Journal of Inflammation. 10.1177/2058739218818956
Wang Yanxiu et al. (2011) Analgesic effect of diprospan in rats with trigeminal neuralgia. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-011-0388-1
