| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Non-muscle caldesmon;CDM;L-caldesmon;Cald1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human uterus smooth muscle extract. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) is an antibody targeting CALD1. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: hHCD; isotype: Mouse IgG1; reactivity: Human,Mouse,Rabbit; observed MW: 61 kDa; calculated MW: 60584 MW.
Boster Bio Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) catalog # MA1009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit.
Key elements and design rationale
- Target: CALD1 — Non-muscle caldesmon
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: hHCD; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rabbit
- Molecular weight guidance: Observed: 61 kDa; Calculated: 60584 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration. .
Scientific background (datasheet): Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al. (1992) demonstrated that the human CDM gene is composed of 14 exons.
Cellular localization (datasheet): Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .
Tissue details (datasheet): High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Sequence similarities (datasheet): Belongs to the calcineurin regulatory subunit family.
Research relevance and current trends
- Commonly studied in contexts related to Actin Binding Proteins,Actin, etc.,Cardiovascular,Contractility,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
