| Field | Specification |
|---|---|
| Target | |
| Alternative names | Non-muscle caldesmon;CDM;L-caldesmon;Cald1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Human uterus smooth muscle extract. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone hHCD is a mouse monoclonal antibody that recognizes non-muscle caldesmon (gene Cald1) in human, mouse and rabbit samples and is validated for Western blot and IHC.
Caldesmon (CALD1) is an actin- and myosin-binding protein that regulates actomyosin interaction. The high-molecular-weight isoform is characteristic of smooth muscle, where it inhibits actomyosin ATPase activity, while the low-molecular-weight isoform acts in nonmuscle cells on stress fibers and cell motility. It is commonly used as a smooth muscle marker.
| Target | Non-muscle caldesmon (gene Cald1; UniProt Q62736, rat) |
|---|---|
| Clone | hHCD |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human, Mouse, Rabbit |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | Store at −20 °C for up to 12 months or 4 °C for up to 1 month from receipt. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rabbit) |
|---|---|
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rabbit) |
Immunogen
Human uterus smooth muscle extract.
Reactivity Notes
The supplier lists reactivity with human, mouse and rabbit. UniProt places non-muscle caldesmon in the cytoplasm. Tissue expression noted by UniProt (rat): High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
