| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calponin-2;Calponin H2, smooth muscle;Neutral calponin;Cnn2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Turkey gizzard calponin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Calponin Cnn2 Antibody (Monoclonal, CP-93) is an antibody targeting CNN2. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: CP-93; isotype: Mouse IgG1; reactivity: Chicken,Human,Turkey; observed MW: 33 kDa; calculated MW: 33156 MW.
Boster Bio Anti-Calponin Cnn2 Antibody (Monoclonal, CP-93) catalog # MA1011. Tested in IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Turkey.
Key elements and design rationale
- Target: CNN2 — Calponin-2
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: CP-93; Isotype: Mouse IgG1
- Species reactivity: Chicken,Human,Turkey
- Molecular weight guidance: Observed: 33 kDa; Calculated: 33156 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Scientific background (datasheet): Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
Cellular localization (datasheet): Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. .
Tissue details (datasheet): Smooth muscle, and tissues containing significant amounts of smooth muscle.
Sequence similarities (datasheet): Belongs to the calponin family.
Research relevance and current trends
- Commonly studied in contexts related to Actin Binding Proteins,Actin, etc.,Cardiovascular,Contractility,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
