| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Carboxypeptidase B2;3.4.17.20 ;Carboxypeptidase U;CPU;Plasma carboxypeptidase B;pCPB;Thrombin-activable fibrinolysis inhibitor;TAFI;CPB2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CPB2 recombinant protein (Position: K166-D388). Human CPB2 shares 86.1% and 85.7% amino acid (aa) sequence identity with mouse and rat CPB2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CPB2 (Carboxypeptidase B2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Carboxypeptidase B2/CPB2 Antibody Picoband® catalog # PB9997. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CPB2 recombinant protein (Position: K166-D388). Human CPB2 shares 86.1% and 85.7% amino acid (aa) sequence identity with mouse and rat CPB2, respectively. (reported region: K166-D388).
- Molecular weight context: reported MW: 48 kDa, 60 kDa; calculated MW: 48424 MW
- Reactivity: Human
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Carboxypeptidase B2; Carboxypeptidase B2. Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen. Functional note: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. . Reported localization: Secreted. Expression/tissue context: Plasma; synthesized in the liver.
Research relevance and current trends
- Amino Acid Metabolism: Researchers commonly examine how CPB2 (Carboxypeptidase B2) relates to this theme using model systems and orthogonal readouts.
- Amino Acids: Researchers commonly examine how CPB2 (Carboxypeptidase B2) relates to this theme using model systems and orthogonal readouts.
- Atherosclerosis: Researchers commonly examine how CPB2 (Carboxypeptidase B2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CPB2 (Carboxypeptidase B2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CPB2 (Carboxypeptidase B2) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
