| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived rat Cardiac FABP recombinant protein (Position: A2-A133). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Fabp3 (activating transcription factor 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4) catalog # M01734. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Mouse Monoclonal Mouse IgG1
- Clone number: Clone: 6C4
- Immunogen / epitope context: E. coli-derived rat Cardiac FABP recombinant protein (Position: A2-A133). (reported region: A2-A133).
- Molecular weight context: reported MW: 15 kDa; calculated MW: nan
- Reactivity: Mouse,Rat
- Applications: ELISA, IHC, WB
As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.
Biological background
activating transcription factor 1; fatty acid binding protein 3. Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP. Functional note: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Reported localization: Cytoplasm. Expression/tissue context: Heart, but also skeletal muscle, kidney, brain and mammary gland.
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how Fabp3 (activating transcription factor 1) relates to this theme using model systems and orthogonal readouts.
- Fatty Acid Oxidation: Researchers commonly examine how Fabp3 (activating transcription factor 1) relates to this theme using model systems and orthogonal readouts.
- Fatty Acids: Researchers commonly examine how Fabp3 (activating transcription factor 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Fabp3 (activating transcription factor 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Fabp3 (activating transcription factor 1) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
