| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Junction plakoglobin;Jup; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant chicken plakoglobin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Catenin Gamma (Plakoglobin) Jup Antibody (Monoclonal, 15F11) is an antibody targeting JUP. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: 15F11; isotype: Mouse IgG1; reactivity: Bovine,Human; observed MW: 82 kDa; calculated MW: 81801 MW.
Boster Bio Anti-Catenin Gamma (Plakoglobin) Jup Antibody (Monoclonal, 15F11) catalog # MA1012. Tested in IHC, ICC, WB applications. This antibody reacts with Bovine, Human.
Key elements and design rationale
- Target: JUP — Junction plakoglobin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: 15F11; Isotype: Mouse IgG1
- Species reactivity: Bovine,Human
- Molecular weight guidance: Observed: 82 kDa; Calculated: 81801 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). .
Scientific background (datasheet): Junction Plakoglobin (JUP) ,also knows as catenin gamma, is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. It is a component of the cadherin-catenin complex, which is predominantly localized where actin filaments anchor in adherens junctions of epithelial cells. The human plakoglobin gene localizes on chromosome 17q21. Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin.
Cellular localization (datasheet): Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form. .
Tissue details (datasheet): Smooth muscle, and tissues containing significant amounts of smooth muscle.
Sequence similarities (datasheet): Belongs to the beta-catenin family.
Research relevance and current trends
- Commonly studied in contexts related to Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
