| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cathepsin L1;3.4.22.15;Cathepsin L;Cyclic protein 2;CP-2;Major excreted protein;MEP;Procathepsin L;Cathepsin L1 heavy chain;Cathepsin L1 light chain;Ctsl;Ctsl1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Procathepsin L isolated from the human lung cancer cell line EPLC 32M1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) is an antibody targeting CTSL1. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: 33/2; isotype: Mouse IgG1; reactivity: Human,Mouse,Rat; observed MW: 25 kDa; calculated MW: 37660 MW.
Boster Bio Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) catalog # MA1014. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CTSL1 — Cathepsin L1
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: 33/2; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 25 kDa; Calculated: 37660 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Important for the overall degradation of proteins in lysosomes. Procathepsin L is required for maximal stimulation of steroidogenesis by TIMP1. .
Scientific background (datasheet): Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.
Cellular localization (datasheet): Lysosome .
Tissue details (datasheet): Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
Sequence similarities (datasheet): Belongs to the peptidase C1 family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Membrane Proteins,Neuroscience,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
