| Field | Specification |
|---|---|
| Target | |
| Alternative names | Monocyte chemoattractant protein 2,Small-inducible cytokine A8,HC14,C-C motif chemokine 8,SCYA10,MCP2,SCYA8,CCL8,Monocyte chemotactic protein 2,MCP-2 |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli - derived recombinant Human CCL8/MCP-2 (Ser29-Pro99). |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Antibody against CCL8 (Monocyte chemoattractant protein 2) for research detection of protein expression and localization.
Target biology
CCL8 encodes a chemokine that directs leukocyte trafficking via gradients sensed by chemokine receptors. Chemokines are central to inflammation and immune-cell recruitment, and are commonly profiled in infection, autoimmunity and tumor microenvironment studies.
Expected localization
Secreted; can associate with extracellular matrix and cell surfaces.
Research context
This target is frequently studied in the context of Immunology & Inflammation research. Application suitability (e.g., ELISA, IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human).
Recommended experimental notes
- For Western blotting, optimize lysis conditions and include reducing/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.
- For IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive/negative tissues.
- For ELISA, ensure capture/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.
Controls & interpretation
Where possible, confirm specificity using genetic perturbation (KO/KD/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low/none of CCL8, and a positive control sample known to express CCL8.
Immunogen context
Generated using an immunogen derived from E. coli - derived recombinant Human CCL8/MCP-2 (Ser29-Pro99).. When interpreting results, note that epitope accessibility can vary with denaturation, fixation, or post‑translational modifications; empirical optimization is recommended.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
