Anti-CD11b ITGAM Rabbit Monoclonal Antibody

Overview

Anti-CD11b ITGAM Rabbit Monoclonal Antibody is an antibody targeting ITGAM. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: BCG-9; isotype: Rabbit IgG; reactivity: Human; observed MW: 95 kDa; calculated MW: 127179 MW.

Boster Bio Anti-CD11b ITGAM Rabbit Monoclonal Antibody catalog # M00144. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: ITGAM — Integrin alpha-M
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: BCG-9; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 95 kDa; Calculated: 127179 MW

Biological background

Protein function (datasheet): Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.

Tissue details (datasheet): Predominantly expressed in monocytes and granulocytes.

Research relevance and current trends

• Commonly studied in contexts related to Cell Adhesion,Cell Type Marker,Cell Type Markers,Cytoskeleton/ECM,Hematopoietic Progenitors,Integrins,Lymphoid,Mesenchymal Stem Cells,Myeloid,Neuroscience,Signal Transduction,Stem Cells,T Lymphocytic Lineage.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.