| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Monocyte differentiation antigen CD14 ;Myeloid cell-specific leucine-rich glycoprotein;CD14;Monocyte differentiation antigen CD14, urinary form;Monocyte differentiation antigen CD14, membrane-bound form;CD14; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD14 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD14 Rabbit Monoclonal Antibody is an antibody targeting CD14. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: FID-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 25 kDa; calculated MW: 40076 MW.
Boster Bio Anti-CD14 Rabbit Monoclonal Antibody catalog # M00137. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD14 — Monocyte differentiation antigen CD14
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: FID-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 25 kDa; Calculated: 40076 MW
Biological background
Protein function (datasheet): Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL (-)) and mediates the cytokine release induced by LDL (-) (PubMed:23880187). .
Cellular localization (datasheet): Cell membrane ; Lipid- anchor, GPI-anchor . Secreted . Membrane raft . Golgi apparatus . Secreted forms may arise by cleavage of the GPI anchor. .
Tissue details (datasheet): Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Type Markers,Hematopoietic Progenitors,Immunology,Innate Immunity,Mesenchymal Stem Cells,Myeloid,Stem Cells,TLR Signaling.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
